KNLTTP_Môi trường PCA và ISPA

Plate Count Agar (PCA)

Direction:

Suspend 23,5g in 1000ml distiled water, boil to dissolve th medium completely. Sterilize by autoclaving at 15 lbs pressure (121^oC) for 15 minute.

Standard formula:

Casein enzymic hydrolysate 5 g/l
Yeast extract 2,5 g/l
Dextrose 1 g/l
Agar 15 g/l
Final pH (at 25^oC) 7,0

Use:

For the determination of plate counts of microorganisms in foods, water and wast water.

Iron Sulphite Argar

Directions:

Suspend 26 gram in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121^oC) for 15 minutes. Mix well an pour into sterile petri plates.

Standard formula:

Casein enzymic hydrolysate 10 g/l
Sodium sulphate 0,5 g/l
Iron citrate 0,5 g/l
Agar 15 g/l
Final pH (at 25^oC) 7,1

Use:

For detection of thermophilic anaerobic organisms causing sulphide spoilage in foods